Header Graphic




  Supporting the Sustainable Management of Amphibian and Reptile Biodiversity

"Never doubt that a small group of thoughtful, committed people can
change the world. Indeed, it is the only thing that ever has." --Anon.



Reproduction Technologies for Amphibians

Reproduction technologies (RTs) for amphibians have now reached the stage where they con provide for the more cost efficient and secure management of Conservation Breeding Programs (CBPs), the perpetuation of amphibian genetic variation, and for DNA related conservation initiatives. This facility is mainly provided through the ability to cryopreserve amphibian sperm and its use to produce offspring in CBS and by the provision of genetic material for a range of conservation purposes.

The use of reproduction technologies for amphibian conservation can be broadly divided into; 1) the hormonal induction of sperm and oocytes (unfertilised eggs), 2) the short term storage of sperm at refrigeration temperatures above freezing, 3) the long term storage of sperm by cryopreservation, 4) artificial fertilisation either by simply mixing sperm and oocytes, and 5) the injection of sperm into oocytes (ICSI - Intracytoplasmic sperm injection.

Here we provide references and analysis of research directly related to the cryopreservation of amphibian sperm and research output over time. Sperm cryopreservation for amphibians.



 Amphibian and Reptile Conservation logo


 Dr Robert Browne

Dr. Robert Browne Chairperson

Dr. Browne established the Internet based ARC in 2011 and expanded it globally in 2013. Robert is committed to achieving the ARC's goal to provide for the sustainable management of amphibians and reptiles. He has a wide international experience in herpetological conservation and has published over 40 scientific articles on amphibian and reptile conservation. see Biography